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real assembly talen kit  (Addgene inc)


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    Structured Review

    Addgene inc real assembly talen kit
    Real Assembly Talen Kit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/real+assembly+talen+kit/REAL+Assembly+TALEN+Kit+(Kit+%231000000017)/bio_rxiv__2025__05__10__653271-236-21-25
    Average 93 stars, based on 33 article reviews
    real assembly talen kit - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Construct:

    Article Title: Spatio-temporal regulation of concurrent developmental processes by generic signaling downstream of chemokine receptors
    Article Snippet: .. The sequences of ccr7 TALEN targets are 5’TCCAACATGACTGAACAC and 5’TCATACTCTGTTGTAG, designed by ZiFit ( http://zifit.partners.org/ZiFiT/ ) ( ) and TALEN plasmids were constructed using REAL Assembly TALEN Kit (Addgene TALEN kit 1000000017) as described previously ( ; ). .. The TALEN RNAs were synthesized using SP6 mMessageMachine Kit (Ambion) and injected into the one-cell stage eggs with 20–50 pg of each RNA.

    Article Title: Spatio-temporal regulation of concurrent developmental processes by generic signaling downstream of chemokine receptors
    Article Snippet: .. The sequences of ccr7 TALEN targets are 5’TCCAACATGACTGAACAC and 5’TCATACTCTGTTGTAG, designed by ZiFit (http://zifit.partners. org/ZiFiT/) (Sander et al., 2011) and TALEN plasmids were constructed using REAL Assembly TALEN Kit (Addgene TALEN kit 1000000017) as described previously (Sander et al., 2011; Shin et al., 2014). .. The TALEN RNAs were synthesized using SP6 mMessageMachine Kit (Ambion) and injected into the one-cell stage eggs with 20–50 pg of each RNA.

    Article Title: Generation of a heterozygous p53 R249S mutant human embryonic stem cell line by TALEN-mediated genome editing.
    Article Snippet: The pair of homologous arms were then digested with EcoRI (left homologous arm), and BamHI and NotI (right homologous arm), and subcloned into the pFNF vector (Addgene #22687), which introduced a FNF selection cassette (Frt-EM7-NeoR-Frt) into intron of the TP53 genomic region to facilitate clone selection. .. The paired TALEN constructs (targeting 5′-tccaggtcaggagccact-3′ upstream and 5′-ccggacgacacggggt-3′ downstream) were designed by ZiFiT Targeter software (http://zifit.partners.org/ZiFiT/ TALZiFiTNuclease.aspx) and then assembled using the REAL Assembly TALEN kit (Addgene) and ligated into the JDS74 vector (Addgene #32288) following the manufacture's protocol (Sander et al., 2011) to generate the TALEN vectors, in which Fok1 nucleases were fused to engineered TALEN domains to induce a double strand break (DSB) precisely at the targeted endogenous genomic location. .. The pCAGGS-flpE-puro plasmid (Addgene #20733) carrying Flp recombinase was used to excise the FNF cassette by transfection.

    Subcloning:

    Article Title: CXCR3-CXCL11 Signaling Restricts Angiogenesis and Promotes Pericyte Recruitment
    Article Snippet: .. The assembly of RVD (repeat variable di-residue)-containing repeats and subcloning of TALE repeats into modified TALE nuclease expression vectors was performed using the REAL Assembly TALEN Kit (No. 1000000017; Addgene), following the previously described cloning steps with small modification. .. After TALEN construction, TALEN RNAs were synthesized using SP6 mMessage mMachine Kit (No. AM1340; Ambion) and purified by Micro Bio-Spin P-30 Gel columns (Bio-Rad) and injected into the cytosol of early 1-cell zygotes.

    Article Title: Regulation of the Yolk Microtubule and Actin Cytoskeleton by Dachsous Cadherins during Zebrafish Epiboly
    Article Snippet: .. The process involved in assembling RVD-containing repeats and subcloning TALE repeats into modified TALE nuclease expression vectors was performed using the REAL Assembly TALEN Kit (Addgene TALEN kit 1000000017) (Sander et al., 2011). .. TALEN RNAs were synthesized using SP6 mMessage mMachine Kit (Ambion) and purified by Micro Bio-SpinTM P-30 Gel columns (Bio-Rad).

    Article Title: CXCR3-CXCL11 Signaling Restricts Angiogenesis and Promotes Pericyte Recruitment
    Article Snippet: .. The assembly of RVD-containing repeats and subcloning of TALE repeats into modified TALE nuclease expression vectors was performed using the REAL Assembly TALEN Kit (No. 1000000017; Addgene), following the previously described cloning steps32 with small modification.33 After TALEN construction, TALEN RNAs were synthesized using SP6 mMessage mMachine Kit (No. AM1340; Ambion) and purified by Micro Bio-Spin P-30 Gel columns (Bio-Rad) and injected into the cytosol of early 1-cell zygotes. ..

    Modification:

    Article Title: CXCR3-CXCL11 Signaling Restricts Angiogenesis and Promotes Pericyte Recruitment
    Article Snippet: .. The assembly of RVD (repeat variable di-residue)-containing repeats and subcloning of TALE repeats into modified TALE nuclease expression vectors was performed using the REAL Assembly TALEN Kit (No. 1000000017; Addgene), following the previously described cloning steps with small modification. .. After TALEN construction, TALEN RNAs were synthesized using SP6 mMessage mMachine Kit (No. AM1340; Ambion) and purified by Micro Bio-Spin P-30 Gel columns (Bio-Rad) and injected into the cytosol of early 1-cell zygotes.

    Article Title: Regulation of the Yolk Microtubule and Actin Cytoskeleton by Dachsous Cadherins during Zebrafish Epiboly
    Article Snippet: .. The process involved in assembling RVD-containing repeats and subcloning TALE repeats into modified TALE nuclease expression vectors was performed using the REAL Assembly TALEN Kit (Addgene TALEN kit 1000000017) (Sander et al., 2011). .. TALEN RNAs were synthesized using SP6 mMessage mMachine Kit (Ambion) and purified by Micro Bio-SpinTM P-30 Gel columns (Bio-Rad).

    Article Title: CXCR3-CXCL11 Signaling Restricts Angiogenesis and Promotes Pericyte Recruitment
    Article Snippet: .. The assembly of RVD-containing repeats and subcloning of TALE repeats into modified TALE nuclease expression vectors was performed using the REAL Assembly TALEN Kit (No. 1000000017; Addgene), following the previously described cloning steps32 with small modification.33 After TALEN construction, TALEN RNAs were synthesized using SP6 mMessage mMachine Kit (No. AM1340; Ambion) and purified by Micro Bio-Spin P-30 Gel columns (Bio-Rad) and injected into the cytosol of early 1-cell zygotes. ..

    Expressing:

    Article Title: CXCR3-CXCL11 Signaling Restricts Angiogenesis and Promotes Pericyte Recruitment
    Article Snippet: .. The assembly of RVD (repeat variable di-residue)-containing repeats and subcloning of TALE repeats into modified TALE nuclease expression vectors was performed using the REAL Assembly TALEN Kit (No. 1000000017; Addgene), following the previously described cloning steps with small modification. .. After TALEN construction, TALEN RNAs were synthesized using SP6 mMessage mMachine Kit (No. AM1340; Ambion) and purified by Micro Bio-Spin P-30 Gel columns (Bio-Rad) and injected into the cytosol of early 1-cell zygotes.

    Article Title: Regulation of the Yolk Microtubule and Actin Cytoskeleton by Dachsous Cadherins during Zebrafish Epiboly
    Article Snippet: .. The process involved in assembling RVD-containing repeats and subcloning TALE repeats into modified TALE nuclease expression vectors was performed using the REAL Assembly TALEN Kit (Addgene TALEN kit 1000000017) (Sander et al., 2011). .. TALEN RNAs were synthesized using SP6 mMessage mMachine Kit (Ambion) and purified by Micro Bio-SpinTM P-30 Gel columns (Bio-Rad).

    Article Title: CXCR3-CXCL11 Signaling Restricts Angiogenesis and Promotes Pericyte Recruitment
    Article Snippet: .. The assembly of RVD-containing repeats and subcloning of TALE repeats into modified TALE nuclease expression vectors was performed using the REAL Assembly TALEN Kit (No. 1000000017; Addgene), following the previously described cloning steps32 with small modification.33 After TALEN construction, TALEN RNAs were synthesized using SP6 mMessage mMachine Kit (No. AM1340; Ambion) and purified by Micro Bio-Spin P-30 Gel columns (Bio-Rad) and injected into the cytosol of early 1-cell zygotes. ..

    Cloning:

    Article Title: CXCR3-CXCL11 Signaling Restricts Angiogenesis and Promotes Pericyte Recruitment
    Article Snippet: .. The assembly of RVD (repeat variable di-residue)-containing repeats and subcloning of TALE repeats into modified TALE nuclease expression vectors was performed using the REAL Assembly TALEN Kit (No. 1000000017; Addgene), following the previously described cloning steps with small modification. .. After TALEN construction, TALEN RNAs were synthesized using SP6 mMessage mMachine Kit (No. AM1340; Ambion) and purified by Micro Bio-Spin P-30 Gel columns (Bio-Rad) and injected into the cytosol of early 1-cell zygotes.

    Article Title: CXCR3-CXCL11 Signaling Restricts Angiogenesis and Promotes Pericyte Recruitment
    Article Snippet: .. The assembly of RVD-containing repeats and subcloning of TALE repeats into modified TALE nuclease expression vectors was performed using the REAL Assembly TALEN Kit (No. 1000000017; Addgene), following the previously described cloning steps32 with small modification.33 After TALEN construction, TALEN RNAs were synthesized using SP6 mMessage mMachine Kit (No. AM1340; Ambion) and purified by Micro Bio-Spin P-30 Gel columns (Bio-Rad) and injected into the cytosol of early 1-cell zygotes. ..

    TALENs:

    Article Title: A homozygous p53 R282W mutant human embryonic stem cell line generated using TALEN-mediated precise gene editing.
    Article Snippet: .. TALENs targeting 5′-TTTCCTTACTGCCTCT-3′ upstream, and 5′-CTATCCTGAGTAGTGGTA3′ downstream were assembled using REAL Assembly TALEN kit (Addgene) and inserted into JDS vectors according to protocol (Sander et al. 2011). .. Homologous arms were amplified using H1 hESC genomic DNA isolated by the PureLink Genomic DNA Mini Kit (Thermo Fisher).

    Article Title: Generation of corrected-hiPSC (USTCi001-A-1) from epilepsy patient iPSCs using TALEN-mediated editing of the SCN1A gene.
    Article Snippet: When cells reached 70–90% confluence, they were passaged (1:3 split) every 3 days using 0.5 mM EDTA (Sigma) solution. .. TALENs targeting 5′-GATGCAATGGCTAGTCAG-3′ upstream, and 5′-CTACGTGTTACCGA TCAGTC-3′ downstream were assembled using REAL Assembly TALEN kit (Addgene) according to manufacturer's instructions. .. Homologous arms were amplified using normal hiPSCs genomic DNA isolated by TIANamp Genomic DNA Kit (TIANGEN) (Lei et al. (2012)).

    Synthesized:

    Article Title: CXCR3-CXCL11 Signaling Restricts Angiogenesis and Promotes Pericyte Recruitment
    Article Snippet: .. The assembly of RVD-containing repeats and subcloning of TALE repeats into modified TALE nuclease expression vectors was performed using the REAL Assembly TALEN Kit (No. 1000000017; Addgene), following the previously described cloning steps32 with small modification.33 After TALEN construction, TALEN RNAs were synthesized using SP6 mMessage mMachine Kit (No. AM1340; Ambion) and purified by Micro Bio-Spin P-30 Gel columns (Bio-Rad) and injected into the cytosol of early 1-cell zygotes. ..

    Purification:

    Article Title: CXCR3-CXCL11 Signaling Restricts Angiogenesis and Promotes Pericyte Recruitment
    Article Snippet: .. The assembly of RVD-containing repeats and subcloning of TALE repeats into modified TALE nuclease expression vectors was performed using the REAL Assembly TALEN Kit (No. 1000000017; Addgene), following the previously described cloning steps32 with small modification.33 After TALEN construction, TALEN RNAs were synthesized using SP6 mMessage mMachine Kit (No. AM1340; Ambion) and purified by Micro Bio-Spin P-30 Gel columns (Bio-Rad) and injected into the cytosol of early 1-cell zygotes. ..

    Injection:

    Article Title: CXCR3-CXCL11 Signaling Restricts Angiogenesis and Promotes Pericyte Recruitment
    Article Snippet: .. The assembly of RVD-containing repeats and subcloning of TALE repeats into modified TALE nuclease expression vectors was performed using the REAL Assembly TALEN Kit (No. 1000000017; Addgene), following the previously described cloning steps32 with small modification.33 After TALEN construction, TALEN RNAs were synthesized using SP6 mMessage mMachine Kit (No. AM1340; Ambion) and purified by Micro Bio-Spin P-30 Gel columns (Bio-Rad) and injected into the cytosol of early 1-cell zygotes. ..

    Software:

    Article Title: Generation of a heterozygous p53 R249S mutant human embryonic stem cell line by TALEN-mediated genome editing.
    Article Snippet: The pair of homologous arms were then digested with EcoRI (left homologous arm), and BamHI and NotI (right homologous arm), and subcloned into the pFNF vector (Addgene #22687), which introduced a FNF selection cassette (Frt-EM7-NeoR-Frt) into intron of the TP53 genomic region to facilitate clone selection. .. The paired TALEN constructs (targeting 5′-tccaggtcaggagccact-3′ upstream and 5′-ccggacgacacggggt-3′ downstream) were designed by ZiFiT Targeter software (http://zifit.partners.org/ZiFiT/ TALZiFiTNuclease.aspx) and then assembled using the REAL Assembly TALEN kit (Addgene) and ligated into the JDS74 vector (Addgene #32288) following the manufacture's protocol (Sander et al., 2011) to generate the TALEN vectors, in which Fok1 nucleases were fused to engineered TALEN domains to induce a double strand break (DSB) precisely at the targeted endogenous genomic location. .. The pCAGGS-flpE-puro plasmid (Addgene #20733) carrying Flp recombinase was used to excise the FNF cassette by transfection.

    Plasmid Preparation:

    Article Title: Generation of a heterozygous p53 R249S mutant human embryonic stem cell line by TALEN-mediated genome editing.
    Article Snippet: The pair of homologous arms were then digested with EcoRI (left homologous arm), and BamHI and NotI (right homologous arm), and subcloned into the pFNF vector (Addgene #22687), which introduced a FNF selection cassette (Frt-EM7-NeoR-Frt) into intron of the TP53 genomic region to facilitate clone selection. .. The paired TALEN constructs (targeting 5′-tccaggtcaggagccact-3′ upstream and 5′-ccggacgacacggggt-3′ downstream) were designed by ZiFiT Targeter software (http://zifit.partners.org/ZiFiT/ TALZiFiTNuclease.aspx) and then assembled using the REAL Assembly TALEN kit (Addgene) and ligated into the JDS74 vector (Addgene #32288) following the manufacture's protocol (Sander et al., 2011) to generate the TALEN vectors, in which Fok1 nucleases were fused to engineered TALEN domains to induce a double strand break (DSB) precisely at the targeted endogenous genomic location. .. The pCAGGS-flpE-puro plasmid (Addgene #20733) carrying Flp recombinase was used to excise the FNF cassette by transfection.



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